Recombinant Human CD52 Protein (Gly25-Ser36), C-hFc-tagged

Product Description

Cat

IMP-10341

Official Symbol

CD52

Product Overview

Recombinant human CD52 protein (Gly25-Ser36) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.

Description

Involved in positive regulation of cytosolic calcium ion concentration. Predicted to be located in extracellular region and plasma membrane. Predicted to be intrinsic component of plasma membrane. Predicted to be active in sperm midpiece.

Expression System

HEK293

Species

Human

Tag

C-hFc

Predicted N Terminal

Gly25

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 28 kDa
SDS-PAGE: 38-44 kDa, reducing conditions

Protein length

Gly25-Ser36

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD52 Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human Siglec-10 Fc Chimera that produces 50% of the optimal binding response is approximately 1-5 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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