Recombinant Human CD55 Protein (Asp35-Ser353), C-6×His-tagged
Product Description
Cat
IMP-10329
Official Symbol
CD55
Product Overview
Recombinant human CD55/DAF protein (Asp35-Ser353) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
Asp35
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 36.0 kDa
SDS-PAGE: 61-75 kDa, reducing conditions
Protein length
Asp35-Ser353
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) CD55/DAF is coated at 1 μg/mL (100 μL/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.5-2.5 μg/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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