Recombinant Human CD59 Protein (Leu26-Asn102), C-10×His-tagged
Product Description
Cat
IMP-10318
Official Symbol
CD59
Product Overview
Recombinant human CD59 protein (Leu26-Asn102) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-10×His
Predicted N Terminal
Starts at Leu26
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 10.3 kDa
SDS-PAGE: 10-20 kDa, reducing conditions
Protein length
Leu26-Asn102
Bio-activity
Measured by its binding ability in a functional ELISA. When recombinant human C9 is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Human CD59 that produces 50% optimal binding response is 0.6-3 μg/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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