Recombinant Human CD74 Protein (Gln73-Met232), N-HA-tagged
Product Description
Cat
IMP-10324
Official Symbol
CD74
Product Overview
Recombinant human CD74 protein (Gln73-Met232) with an N-terminal HA (YPYDVPDYA) tag was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Expression System
CHO
Species
Human
Tag
N-HA
Predicted N Terminal
Tyr
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 19.3 kDa
SDS-PAGE: 30-35 kDa, reducing conditions
Protein length
Gln73-Met232
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When 5 x 104 cells per well are added to Recombinant Human CD74 coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.6-3 μg/mL. Optimal dilutions should be determined by each laboratory for each application.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info