Recombinant Human CD80 protein, C-Fc Tag

Product Description

Cat

IMP-268

Official Symbol

CD80

Product Overview

Recombinant Human CD80 protein(AA Val 35-Asn 242)(Accession # NP_005182.1) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.0 kDa. The protein migrates as 65-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Val 35-Asn 242

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human B7-1, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HPLC

The purity of Human B7-1, Fc Tag (HPLC-verified) was greater than 95% as determined by SEC-HPLC.

Bioactivity-ELISA 1

Immobilized Human B7-1, Fc Tag (HPLC-verified) at 2 ug/mL (100 uL/well) can bind Human CTLA-4, His Tag with a linear range of 0.1-2 ng/mL.

Bioactivity-ELISA 2

Immobilized Human B7-1, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag with a linear range of 0.02-1.25 ug/mL.

Bioactivity-ELISA 3

Immobilized Human B7-1, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Biotinylated Human CTLA-4, Fc,Avitag with a linear range of 0.6-10 ng/mL.

Bioactivity-ELISA 4

Immobilized Biotinylated Mouse CD28, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Human B7-1, Fc Tag (HPLC-verified) with a linear range of 0.2-2 ng/mL.

Bioactivity-ELISA 5

Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Human B7-1, Fc Tag (HPLC-verified) with a linear range of 0.2-5 ng/mL.

Bioactivity-BLI 1

Loaded Human B7-1, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag with an affinity constant of 5.1 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human B7-1, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human CTLA-4, His Tag, active dimer with an affinity constant of 0.498 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

FACS assay shows that Human B7-1, Fc Tag (HPLC-verified) can bind to 293T cells overexpressing human CTLA4 protein. The concentration of Human B7-1 is 1 ug/mL.

Bioactivity-SPR 1

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, Fc Tag (HPLC-verified) with an affinity constant of 2.01 uM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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