Recombinant Human CD80 protein, C-His Tag

Product Description

Cat

IMP-166

Official Symbol

CD80

Product Overview

Recombinant Human CD80 protein(AA Val 35-Asn 242)(Accession # P33681-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.8 kDa. The protein migrates as 43-60 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Protein length

Val 35-Asn 242

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human B7-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Human CTLA-4, Fc Tag at 10 ug/mL (100 uL/well) can bind Human B7-1, His Tag with a linear range of 0.04-1.25 ug/mL.

Bioactivity-BLI 1

Loaded Human PD-L1, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 18 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 2.1 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human CTLA-4, Fc Tag on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 0.52 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, His Tag with an affinity constant of 5.29 uM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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