Recombinant Human CD80 protein, C-His Tag

Product Description

Cat

IMP-166

Official Symbol

CD80

Product Overview

Recombinant Human CD80 protein(AA Val 35-Asn 242)(Accession # P33681-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.8 kDa. The protein migrates as 43-60 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Protein length

Val 35-Asn 242

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human B7-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Human CTLA-4, Fc Tag at 10 ug/mL (100 uL/well) can bind Human B7-1, His Tag with a linear range of 0.04-1.25 ug/mL.

Bioactivity-BLI 1

Loaded Human PD-L1, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 18 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 2.1 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human CTLA-4, Fc Tag on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 0.52 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, His Tag with an affinity constant of 5.29 uM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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