Recombinant Human CD80 Protein, C-His&C-AVI-tagged, Biotinylated
Product Description
Cat
IMP-1835
Official Symbol
CD80
Product Overview
Recombinant human CD80 (NP_005182.1)(Met1-Asn242) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Description
The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Expression System
HEK293
Species
Human
Tag
C-His&C-AVI
Predicted N Terminal
Val 35
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human CD80 consists of 234 amino acids and predicts a molecular mass of 27.12 kDa. It migrates as an approximately 48.86 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Asn242
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized human CTLA-4 hFc at 2 μg/mL (100 μL/well) can bind Human B7-1, Biotinylated, the EC50 of Human B7-1, Biotinylated is 60-320 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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