Recombinant Human CD80 protein, C-mFc Tag

Product Description

Cat

IMP-170

Official Symbol

CD80

Product Overview

Recombinant Human CD80 protein(AA Val 35-Asn 242)(Accession # P33681-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 50.9 kDa. The protein migrates as 65-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Val 35-Asn 242

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human B7-1, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human CTLA-4, His Tag at 1 ug/mL (100 uL/well) can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 2-31 ng/mL.

Bioactivity-ELISA 2

Immobilized Human B7-1, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Human PD-L1, Fc Tag with a linear range of 0.01-0.313 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of Ipilimumab were added into Human B7-1, Mouse IgG2a Fc Tag, low endotoxin: Biotinylated Human CTLA-4, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.8402 ug/mL.

Bioactivity-ELISA 4

Immobilized Biotinylated Mouse CD28, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.2-5 ng/mL.

Bioactivity-BLI 1

Loaded Human B7-1, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag with an affinity constant of 4.6 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that Human B7-1, Mouse IgG2a Fc Tag, low endotoxin can bind to 293 cell overexpressing human CTLA-4. The concentration of Human B7-1 is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human B7-1, Mouse IgG2a Fc Tag, low endotoxin to 293 overexpressing CTLA-4 was inhibited by increasing concentration of neutralizing Anti-human CTLA-4 antibody. The concentration of Human B7-1 is 1 ug /mL. The IC50 is 5.5 ug/mL.

Bioactivity-SPR 1

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 2.72 uM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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