Recombinant Human CD80 protein, C-mFc Tag

Product Description

Cat

IMP-170

Official Symbol

CD80

Product Overview

Recombinant Human CD80 protein(AA Val 35-Asn 242)(Accession # P33681-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 50.9 kDa. The protein migrates as 65-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Val 35-Asn 242

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human B7-1, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human CTLA-4, His Tag at 1 ug/mL (100 uL/well) can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 2-31 ng/mL.

Bioactivity-ELISA 2

Immobilized Human B7-1, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Human PD-L1, Fc Tag with a linear range of 0.01-0.313 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of Ipilimumab were added into Human B7-1, Mouse IgG2a Fc Tag, low endotoxin: Biotinylated Human CTLA-4, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.8402 ug/mL.

Bioactivity-ELISA 4

Immobilized Biotinylated Mouse CD28, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.2-5 ng/mL.

Bioactivity-BLI 1

Loaded Human B7-1, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag with an affinity constant of 4.6 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that Human B7-1, Mouse IgG2a Fc Tag, low endotoxin can bind to 293 cell overexpressing human CTLA-4. The concentration of Human B7-1 is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human B7-1, Mouse IgG2a Fc Tag, low endotoxin to 293 overexpressing CTLA-4 was inhibited by increasing concentration of neutralizing Anti-human CTLA-4 antibody. The concentration of Human B7-1 is 1 ug /mL. The IC50 is 5.5 ug/mL.

Bioactivity-SPR 1

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 2.72 uM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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