Recombinant Human CD80 Protein (Val35-Asn242), C-Fc-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10116

Official Symbol

CD80

Product Overview

Alexa Fluor 488 conjugated recombinant human B7-1/CD80 protein (Val35-Asn242) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Expression System

CHO

Species

Human

Tag

C-Fc

Predicted N Terminal

Val35

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 50.5 kDa
SDS-PAGE: 66-80 kDa, under reducing conditions.

Protein length

Val35-Asn242

Bio-activity

Measured by flow cytometry for its ability to bind anti-human B7-1 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human B7-1 Fc-tag Alexa Fluor 488 that produces 50% of the binding response is 30.0-300 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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