Recombinant Human CD80 Protein (Val35-Asn242), C-Fc-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10116

Official Symbol

CD80

Product Overview

Alexa Fluor 488 conjugated recombinant human B7-1/CD80 protein (Val35-Asn242) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Expression System

CHO

Species

Human

Tag

C-Fc

Predicted N Terminal

Val35

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 50.5 kDa
SDS-PAGE: 66-80 kDa, under reducing conditions.

Protein length

Val35-Asn242

Bio-activity

Measured by flow cytometry for its ability to bind anti-human B7-1 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human B7-1 Fc-tag Alexa Fluor 488 that produces 50% of the binding response is 30.0-300 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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