Recombinant Human CD80 Protein (Val35-Asn242), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10315

Official Symbol

CD80

Product Overview

Biotinylated recombinant human B7-1/CD80 protein (Val35-Asn242) with a Human IgG1 Fc tag (Pro100-Lys330) and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Val35

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 52 kDa
SDS-PAGE: 77-89 kDa, under reducing conditions

Protein length

Val35-Asn242

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CTLA-4 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human B7-1/CD80 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 0.4-2.4 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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