Recombinant Human CD80 Protein (Val35-Asn242), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10315

Official Symbol

CD80

Product Overview

Biotinylated recombinant human B7-1/CD80 protein (Val35-Asn242) with a Human IgG1 Fc tag (Pro100-Lys330) and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Val35

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 52 kDa
SDS-PAGE: 77-89 kDa, under reducing conditions

Protein length

Val35-Asn242

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CTLA-4 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human B7-1/CD80 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 0.4-2.4 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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