Recombinant Human CD83 Protein (Thr20-Ala143), C-hFc-tagged
Product Description
Cat
IMP-10308
Official Symbol
CD83
Product Overview
Recombinant human CD83 protein (Thr20-Ala143) with a Human IgG1 (Pro100-Lys330) Fc tag C-terminus was expressed in Spodoptera frugiperda, Sf 21 cells.
Description
The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene.
Expression System
Sf 21 cells
Species
Human
Tag
C-hFc
Predicted N Terminal
Thr20
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 40.7 kDa (monomer)
SDS-PAGE: 51-54 kDa, reducing conditions
Protein length
Thr20-Ala143
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of human monocyte-derived dendritic cells. When 5 × 10^4 cells/well are added to human CD83/Fc Chimera coated plates (5 μg/mL, 100 μL/well), approximately 50-75% will adhere after 30 minutes at 37 centigrade. Optimal dilutions should be determined by each laboratory for each application.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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