Recombinant Human CD84 Protein (Lys22-Arg220), C-10×His-tagged

Product Description

Cat

IMP-10307

Official Symbol

CD84

Product Overview

Recombinant human CD84/SLAMF5 protein (Lys22-Arg220) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-10×His

Predicted N Terminal

Lys22

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 23.6 kDa
SDS-PAGE: 40-43 kDa, reducing conditions

Protein length

Lys22-Arg220

Bio-activity

Measured in a cell proliferation assay using PHA stimulated human T cells in the presence of anti-CD3. Tangye, S.G. et al. (2003) J. Immunol. 171:2485. The ED50 for this effect is 1-4 μg/mL in the presence of anti-CD3 immobilized at least at 20 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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