Recombinant Human CD84 Protein (Lys22-Arg220), C-10×His-tagged

Product Description

Cat

IMP-10307

Official Symbol

CD84

Product Overview

Recombinant human CD84/SLAMF5 protein (Lys22-Arg220) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-10×His

Predicted N Terminal

Lys22

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 23.6 kDa
SDS-PAGE: 40-43 kDa, reducing conditions

Protein length

Lys22-Arg220

Bio-activity

Measured in a cell proliferation assay using PHA stimulated human T cells in the presence of anti-CD3. Tangye, S.G. et al. (2003) J. Immunol. 171:2485. The ED50 for this effect is 1-4 μg/mL in the presence of anti-CD3 immobilized at least at 20 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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