Recombinant Human CD86 Protein (31-247aa), C-His-Tagged
Product Description
Cat
IMP-9504
Official Symbol
CD86
Product Overview
Recombinant Human B7-2/CD86, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description
B7-2/CD86, also known as T-lymphocyte activation antigen CD86 isoform 1, is a variably glycosylated protein in the B7 family. These family members play important roles in immune activation and the maintenance of immune tolerance. It is expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival and exists predominantly as a monomer on cell surfaces. Also, it interacts with two costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells that it induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL.
Expression System
Baculovirus
Species
Human
Tag
C-His
Form
Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Molecular Mass
25.6 kDa (223 aa)
Protein length
31-247
AA Sequence
YFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP<HHHHHH>
Endotoxin
< 1 EU/μg of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Applications
SDS-PAGE
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
0.5 mg/mL (determined by absorbance at 280nm)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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