Recombinant Human CD86 protein, C-Avi-His Tag, Biotinylated
Product Description
Cat
IMP-562
Official Symbol
CD86
Product Overview
Biotinylated Recombinant Human CD86(NP_001193853.1)(Ala24-Pro247) is expressed in HEK293, fused with a Avi, 6His tag at the C-terminus.
Description
The protein is the receptor that involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. It may play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. The protein interacts with MARCH8, human herpesvirus 8 MIR2 protein, adenovirus subgroup B fiber proteins and acts as a receptor for these viruses.It is expressed by activated B-lymphocytes and monocytes and promoted by MARCH8 and results in endocytosis and lysosomal degradation.It contains 1 Ig-like C2-type(immunoglobulin-like) domainand 1 Ig-like V-type (immunoglobulin-like) domain.
Expression System
HEK293
Species
Human
Tag
C-Avi-His Tag
Form
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Molecular Mass
55-80kDa, reducing conditions.
Protein length
Ala24-Pro247
Endotoxin
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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