Recombinant Human CD86 protein, C-Fc Tag

Product Description

Cat

IMP-088

Official Symbol

CD86

Product Overview

Recombinant Human CD86 protein(AA Leu 26-Pro 247)(Accession # AAH40261) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.5 kDa. As a result of glycosylation, the protein migrates as 66-86 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-P

Protein length

Leu 26-Pro 247

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human B7-2, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human B7-2, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human CTLA-4, Fc Tag, Avi Tag with a linear range of 0.6-10 ng/mL.

Bioactivity-ELISA 2

Immobilized Biotinylated Mouse CD28, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Human B7-2, Fc Tag with a linear range of 5-78 ng/mL.

Bioactivity-ELISA 3

Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Human B7-2, Fc Tag with a linear range of 2-78 ng/mL.

Bioactivity-BLI 1

Loaded Human B7-2, Fc Tag on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag with an affinity constant of 4.5 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human B7-2, Fc Tag on Protein A Biosensor, can bind Human CTLA-4, His Tag, active dimer with an affinity constant of 1.03 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that recombinant Human B7-2, Fc Tag can bind to CD28 expressed on Jurkat E6.1. The concentration of B7-2 used is 2 ug/ml.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human B7-2, Fc Tag to CD28 expressed on Jurkat E6.1 was inhibited by increasing concentration of neutralizing anti-CD28 antibody. The concentration of B7-2 used is 2 ug/ml. The IC50 is 0.022 ug/ml.

Bioactivity-FACS 3

FACS assay shows that Human B7-2, Fc Tag can bind to 293T cells overexpressing human CTLA4 protein. The concentration of Human B7-2 is 3 ug/ml.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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