Recombinant Human CD86 protein, C-Fc Tag

Product Description

Cat

IMP-088

Official Symbol

CD86

Product Overview

Recombinant Human CD86 protein(AA Leu 26-Pro 247)(Accession # AAH40261) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.5 kDa. As a result of glycosylation, the protein migrates as 66-86 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-P

Protein length

Leu 26-Pro 247

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human B7-2, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human B7-2, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human CTLA-4, Fc Tag, Avi Tag with a linear range of 0.6-10 ng/mL.

Bioactivity-ELISA 2

Immobilized Biotinylated Mouse CD28, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Human B7-2, Fc Tag with a linear range of 5-78 ng/mL.

Bioactivity-ELISA 3

Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Human B7-2, Fc Tag with a linear range of 2-78 ng/mL.

Bioactivity-BLI 1

Loaded Human B7-2, Fc Tag on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag with an affinity constant of 4.5 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human B7-2, Fc Tag on Protein A Biosensor, can bind Human CTLA-4, His Tag, active dimer with an affinity constant of 1.03 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that recombinant Human B7-2, Fc Tag can bind to CD28 expressed on Jurkat E6.1. The concentration of B7-2 used is 2 ug/ml.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human B7-2, Fc Tag to CD28 expressed on Jurkat E6.1 was inhibited by increasing concentration of neutralizing anti-CD28 antibody. The concentration of B7-2 used is 2 ug/ml. The IC50 is 0.022 ug/ml.

Bioactivity-FACS 3

FACS assay shows that Human B7-2, Fc Tag can bind to 293T cells overexpressing human CTLA4 protein. The concentration of Human B7-2 is 3 ug/ml.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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