Recombinant Human CD86 protein, C-hFc-Avi Tag, Biotinylated

Product Description

Cat

IMP-090

Official Symbol

CD86

Product Overview

Biotinylated Recombinant Human CD86 protein(AA Leu 26-Pro 247)(Accession # AAH40261) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 53.8 kDa. As a result of glycosylation, the protein migrates as 66-100 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Leu 26-Pro 247

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Biotinylated Human B7-2, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human CTLA-4, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human B7-2, Fc,Avitag with a linear range of 19-156 ng/mL.

Bioactivity-ELISA 2

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag: Biotinylated Human B7-2, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.1701 ug/mL.

Bioactivity-FACS 1

Flow Cytometry assay shows that Biotinylated Human B7-2, Fc,Avitag can bind to CD28 expressed on Jurkat E6.1 cells. The concentration of B7-2 used is 1.5 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Biotinylated Human B7-2, Fc,Avitag to CD28 expressed on Jurkat E6.1 was inhibited by increasing concentration of neutralizing Anti-CD28 antibody. The concentration of B7-2 used is 1.5 ug/mL. The IC50 is 0.031 ug/mL.

Bioactivity-FACS 3

Flow Cytometry assay shows that Biotinylated Human B7-2, Fc,Avitag can bind to 293 cell overexpressing human CTLA-4. The concentration of Human B7-2 is 2 ug/mL.

Bioactivity-FACS 4

FACS analysis shows that the binding of Biotinylated Human B7-2, Fc,Avitag to 293 overexpressing CTLA-4 was inhibited by increasing concentration of neutralizing Anti-human CTLA-4 antibody. The concentration of Human B7-2 is 2 ug /mL. The IC50 is 1.014 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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