Recombinant Human CD86 protein, C-His Tag
Product Description
Cat
IMP-089
Official Symbol
CD86
Product Overview
Recombinant Human CD86 protein(AA Leu 26-Pro 247)(Accession # AAH40261) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.
Expression System
HEK293
Species
Human
Tag
C-His Tag
Form
Lyophilized from sterile PBS, pH7.4, 10% trehalose.
Molecular Mass
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.2 kDa. The protein migrates as 42-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Leu 26-Pro 247
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Human B7-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human CTLA-4, mouse IgG2a Fc tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Human B7-2, His Tag with a linear range of 0.01-0.156 ug/mL.

Bioactivity-BLI 1

Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 6.8 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2
Bioactivity-BLI

Loaded Human CTLA-4, Fc Tag on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 1.9 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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