Recombinant Human CD86 protein, C-His Tag

Product Description

Cat

IMP-089

Official Symbol

CD86

Product Overview

Recombinant Human CD86 protein(AA Leu 26-Pro 247)(Accession # AAH40261) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.2 kDa. The protein migrates as 42-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 26-Pro 247

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human B7-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human CTLA-4, mouse IgG2a Fc tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Human B7-2, His Tag with a linear range of 0.01-0.156 ug/mL.

Bioactivity-BLI 1

Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 6.8 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human CTLA-4, Fc Tag on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 1.9 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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