Human B7-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human CTLA-4, mouse IgG2a Fc tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Human B7-2, His Tag with a linear range of 0.01-0.156 ug/mL.
Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 6.8 uM as determined in BLI assay (ForteBio Octet Red96e).
Loaded Human CTLA-4, Fc Tag on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 1.9 uM as determined in BLI assay (ForteBio Octet Red96e).