Recombinant Human CD86 Protein (Leu20-His239), C-Fc-tagged, Alexa Fluor 488 Conjugated
Product Description
Cat
IMP-10112
Official Symbol
CD86
Product Overview
Alexa Fluor 488 conjugated recombinant human B7-2/CD86 protein (Leu20-His239) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm
Description
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Expression System
CHO
Species
Human
Tag
C-Fc
Predicted N Terminal
Leu20
Form
Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Conjugate
Alexa Fluor 488
Molecular Mass
Predicted Molecular Mass: 52 kDa (monomer)
SDS-PAGE: 75-100 kDa, under reducing conditions.
Protein length
Leu20-His239
Bio-activity
Measured by flow cytometry for its ability to bind anti-human B7-2/CD86 Antibody conjugated beads. The concentration of Recombinant Human B7-2/CD86 Fc Chimera Alexa Fluor 488 that produces 50% of the binding response is 50.0-500 ng/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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