Recombinant Human CD86 Protein (Leu20-His239), C-Fc-tagged, Alexa Fluor 647 Conjugated
Product Description
Cat
IMP-10111
Official Symbol
CD86
Product Overview
Alexa Fluor 647 conjugated recombinant human B7-2/CD86 protein (Leu20-His239) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Labeled with Alexa Fluor 647
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm
Description
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Expression System
CHO
Species
Human
Tag
C-Fc
Predicted N Terminal
Leu20
Form
Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Conjugate
Alexa Fluor 647
Molecular Mass
Predicted Molecular Mass: 52 kDa (monomer)
SDS-PAGE: 75-100 kDa, under reducing conditions.
Protein length
Leu20-His239
Bio-activity
Measured by flow cytometry for its ability to bind anti-human B7-2/CD86 Antibody conjugated beads. The concentration of Recombinant Human B7-2/CD86 Fc Chimera Alexa Fluor 647 that produces 50% of the binding response is 2.50-25.0 ng/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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