Recombinant Human CD86 Protein (Leu26-Pro247), C-6×His-tagged, Alexa Fluor 647 Conjugated
Product Description
Cat
IMP-10110
Official Symbol
CD86
Product Overview
Alexa Fluor 647 conjugated recombinant human B7-2/CD86 protein (Leu26-Pro247), with a C-terminal 6×His tag was expressed in human embryonic kidney cell.
Labeled with Alexa Fluor 647
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm
Description
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Expression System
HEK293
Species
Human
Tag
C-6×His
Predicted N Terminal
Leu26
Form
Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Conjugate
Alexa Fluor 647
Molecular Mass
Predicted Molecular Mass: 26 kDa
SDS-PAGE: 57-66 kDa, under reducing conditions.
Protein length
Leu26-Pro247
Bio-activity
Measured by flow cytometry for its ability to bind anti-human B7-2/CD86 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human B7-2/CD86 His-tag Alexa Fluor 647 that produces 50% of the binding response is 6.00-60.0 ng/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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