Recombinant Human CD86 Protein (Leu26-Pro247), C-6×His-tagged, Alexa Fluor 647 Conjugated
Product Description
Cat
IMP-10110
Official Symbol
CD86
Product Overview
Alexa Fluor 647 conjugated recombinant human B7-2/CD86 protein (Leu26-Pro247), with a C-terminal 6×His tag was expressed in human embryonic kidney cell.
Labeled with Alexa Fluor 647
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm
Description
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Expression System
HEK293
Species
Human
Tag
C-6×His
Predicted N Terminal
Leu26
Form
Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Conjugate
Alexa Fluor 647
Molecular Mass
Predicted Molecular Mass: 26 kDa
SDS-PAGE: 57-66 kDa, under reducing conditions.
Protein length
Leu26-Pro247
Bio-activity
Measured by flow cytometry for its ability to bind anti-human B7-2/CD86 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human B7-2/CD86 His-tag Alexa Fluor 647 that produces 50% of the binding response is 6.00-60.0 ng/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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