Recombinant Human CD86 Protein (Leu26-Pro247), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10302
Official Symbol
CD86
Product Overview
Biotinylated recombinant human B7-2/CD86 protein (Leu26-Pro247) with a Human IgG1 Fc tag (Pro100-Lys330) and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Leu26
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 54 kDa
SDS-PAGE: 87-101 kDa, under reducing conditions
Protein length
Leu26-Pro247
Bio-activity
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CTLA-4 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human B7-2/CD86 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 5-30 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CTLA-4 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human B7-2/CD86 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 5-30 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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