Recombinant Human CD86 Protein (Leu26-Pro247), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10302
Official Symbol
CD86
Product Overview
Biotinylated recombinant human B7-2/CD86 protein (Leu26-Pro247) with a Human IgG1 Fc tag (Pro100-Lys330) and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Leu26
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 54 kDa
SDS-PAGE: 87-101 kDa, under reducing conditions
Protein length
Leu26-Pro247
Bio-activity
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CTLA-4 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human B7-2/CD86 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 5-30 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CTLA-4 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human B7-2/CD86 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 5-30 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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