Recombinant Human CD8A Protein (Ser22-Asp182), C-hFc-tagged
Product Description
Cat
IMP-10597
Official Symbol
CD8A
Product Overview
Recombinant human CD8 alpha protein (Ser22-Asp182) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein.
Expression System
CHO
Species
Human
Tag
C-hFc
Predicted N Terminal
Ser22
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 44 kDa
SDS-PAGE: 54-60 kDa, under reducing conditions.
Protein length
Ser22-Asp182
Bio-activity
Measured by its binding ability in a functional ELISA. When Human CD8 alpha Antibody is immobilized at 2.00 μg/mL, 100 μg/mL, Recombinant Human CD8 alpha alpha Fc Chimera binds with an ED50 of 1.50-15.0 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Human CD8 alpha Antibody is immobilized at 2.00 μg/mL, 100 μg/mL, Recombinant Human CD8 alpha alpha Fc Chimera binds with an ED50 of 1.50-15.0 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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