Recombinant Human CD96 protein, C-His Tag

Product Description

Cat

IMP-172

Official Symbol

CD96

Product Overview

Recombinant Human CD96 protein(AA Val 22-Met 503)(Accession # P40200-2) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.4 kDa. The protein migrates as 95-130 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Val 22-Met 503

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CD96, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-BLI 1

SDS-PAGE

Bioactivity-Cell based assay 1

FACS analysis of Human CD96, His Tag binding to CD155 cells overexpressing CD155. 2e5 of CD155 cells overexpressing CD155 were stained with 100 uL of 1 ug/mL of Human CD96, His Tag and negative control protein respectively, washed and then followed by PE anti-His Tag antibody and analyzed with FACS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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