Recombinant Human CD96 protein, C-mFc Tag

Product Description

Cat

IMP-133

Official Symbol

CD96

Product Overview

Recombinant Human CD96 protein(AA Val 22-Met 503)(Accession # P40200-2) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 80.4 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Val 22-Met 503

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>90% as determined by reduced SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CD96, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Human CD96, Mouse IgG2a Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Human CD155, Fc Tag with a linear range of 0.078-1.25 ug/mL.

Bioactivity-Cell based assay 1

FACS analysis of Human CD96, Mouse IgG2a Fc Tag binding to CD155 cells overexpressing CD155. 2e5 of CD155 cells overexpressing CD155 were stained with 100 uL of 1 ug/mL of Human CD96, Mouse IgG2a Fc Tag and negative control protein respectively, washed and then followed by PE-anti-Mouse IgG2a antibody and analyzed with FACS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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