Recombinant Human CD96 protein, C-mFc Tag

Product Description

Cat

IMP-133

Official Symbol

CD96

Product Overview

Recombinant Human CD96 protein(AA Val 22-Met 503)(Accession # P40200-2) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 80.4 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Val 22-Met 503

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>90% as determined by reduced SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CD96, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Human CD96, Mouse IgG2a Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Human CD155, Fc Tag with a linear range of 0.078-1.25 ug/mL.

Bioactivity-Cell based assay 1

FACS analysis of Human CD96, Mouse IgG2a Fc Tag binding to CD155 cells overexpressing CD155. 2e5 of CD155 cells overexpressing CD155 were stained with 100 uL of 1 ug/mL of Human CD96, Mouse IgG2a Fc Tag and negative control protein respectively, washed and then followed by PE-anti-Mouse IgG2a antibody and analyzed with FACS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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