Recombinant Human CD96 Protein (Lys25-Met519), C-hFc-tagged
Product Description
Cat
IMP-10298
Official Symbol
CD96
Product Overview
Recombinant human CD96 protein (Lys25-Met519) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesive interactions of activated T and NK cells during the late phase of the immune response. It may also function in antigen presentation. Alternative splicing generates multiple transcript variants encoding distinct isoforms.
Expression System
HEK293
Species
Human
Tag
C-hFc
Predicted N Terminal
Lys25
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 81 kDa
SDS-PAGE: 131-160 kDa, reducing conditions
Protein length
Lys25-Met519
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD155/PVR Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human CD96 Fc Chimera that produces 50% of the optimal binding response is 0.3-1.8 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD155/PVR Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human CD96 Fc Chimera that produces 50% of the optimal binding response is 0.3-1.8 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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