Recombinant Human CDA Protein (1-146aa), N-His-Tagged

Product Description

Cat

IMP-9329

Official Symbol

CDA

Product Overview

Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Description

CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2- deoxycytidine for uMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes.

Expression System

E. coli

Species

Human

Tag

N-His

Form

Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 2mM EDTA, 100mM NaCl, 40% glycerol

Molecular Mass

18.3 kDa (166 aa) confirmed by MALDI-TOF

Protein length

1-146

AA Sequence

<MGSSHHHHHHSSGLVPRGSH>MAQKRPACTLKPECVQQLLVCSQEAKQSAYCPYSHFPVGAALLTQEGRIFKGCNIENACYPLGICAERTAIQKAVSEGYKDFRAIAIASDMQDDFISPCGACRQVMREFGTNWPVYMTKPDGTYIVMTVQELLPSSFGPEDLQKTQ

Purity

> 90% by SDS-PAGE

Applications

SDS-PAGE, Enzyme Activity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5 mg/mL (determined by Bradford assay)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry