Recombinant Human CEACAM1 Protein (Gln35-Gly428), C-10×His-tagged
Product Description
Cat
IMP-10238
Official Symbol
CEACAM1
Product Overview
Recombinant human CEACAM-1/CD66a protein (Gln35-Gly428) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-10×His
Predicted N Terminal
Gln35
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 44.6 kDa
SDS-PAGE: 100-130 kDa, reducing conditions
Protein length
Gln35-Gly428
Bio-activity
Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells Chen, C.J. and J.E. Shively (2004) J. Immunol. 172:3544. rhCEACAM-1 at concentrations higher than 1 μg/mL reduces anti-CD3 (immobilized at 10 μg/mL) induced IL-2 production by 60-70%. The ED50 for rhCEACAM-1 inhibition of anti-CD3 induced IL-2 production is 0.05-0.2 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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