Recombinant Human CFD Protein, C-His-tagged
Product Description
Cat
IMP-1580
Official Symbol
CFD
Product Overview
Recombinant mature form of human CFD (P00746) (Ile 26-Ala 253) was fused with a C-terminal polyhistidine tag and a signal peptide at the N-terminus.
Description
Complement factor D, also known as Adipsin, C3 convertase activator, Properdin factor D and CFD is a secreted protein which belongs to thepeptidase S1 family. CFD / Adipsin contains onepeptidase S1 domain. Complement factor D (CFD / Adipsin) is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. Complement factor D (CFD / Adipsin) has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Complement factor D (CFD / Adipsin) cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Complement factor D (CFD / Adipsin) is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement factor D deficiency (CFD deficiency) which predisposes to invasive meningococcal disease.
Expression System
HEK293
Species
Human
Tag
C-His
Predicted N Terminal
Ile 26
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The secreted recombinant human CFD consists of 239 amino acids and predicts a molecular mass of 25.8 kDa. As a result of glycosylation, rh CFD migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
Protein length
Ile26-Ala253
Endotoxin
< 1.0 EU/μg protein as determined by the LAL method.
Purity
> 97 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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