Recombinant Human CFD Protein (Ile26-Ala253), C-10×His-tagged
Product Description
Cat
IMP-10653
Official Symbol
CFD
Product Overview
Recombinant human Complement Factor D protein (Ile26-Ala253) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-10×His
Predicted N Terminal
Ile26&His35
Form
Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Molecular Mass
Predicted Molecular Mass: 26 kDa & 25 kDa
SDS-PAGE: 27 kDa & 26 kDa, reducing conditions
Protein length
Ile26-Ala253
Bio-activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >70 pmol/min/μg, as measured under the described conditions.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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