Recombinant Human CFD Protein (Ile26-Ala253), C-10×His-tagged
Product Description
Cat
IMP-10653
Official Symbol
CFD
Product Overview
Recombinant human Complement Factor D protein (Ile26-Ala253) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-10×His
Predicted N Terminal
Ile26&His35
Form
Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Molecular Mass
Predicted Molecular Mass: 26 kDa & 25 kDa
SDS-PAGE: 27 kDa & 26 kDa, reducing conditions
Protein length
Ile26-Ala253
Bio-activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >70 pmol/min/μg, as measured under the described conditions.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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