Immobilized Biotinylated Human Claudin 18.2 VLP on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-Claudin18.2 Antibody, hFc Tag with the EC50 of 62ng/ml determined by ELISA.
Amine coupling SA on CM5 Chip, SA capture Biotinylated Human Claudin 18.2 VLP, Anti-Claudin18.2 Ab. as analyte; The affinity constant of 7.74 nM as determined in SPR assay (Biacore T200).