Immobilized Biotinylated Claudin 6 VLP on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-Claudin 6 Antibody, mFc Tag with the EC50 of 9.5ng/ml determined by ELISA.
Amine coupling SA on CM5 Chip, SA capture Biotinylated Claudin 6 VLP, Anti-Claudin6 Antibody as analyte; The affinity constant of 1.20 nM as determined in SPR assay (Biacore T200).