Recombinant Human CLEC4E Protein (Arg41-Leu219), N-hFc-tagged
Product Description
Cat
IMP-10216
Official Symbol
CLEC4E
Product Overview
Recombinant human CLEC4E protein (Arg41-Leu219) with a Human IgG1 (Pro100-Lys330) Fc tag at N-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.
Expression System
CHO
Species
Human
Tag
N-hFc
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 47 kDa
SDS-PAGE: 54-64 kDa, reducing conditions
Protein length
Arg41-Leu219
Bio-activity
Measured by its binding ability in a functional ELISA. When Trehalose 6, 6'-Dimycolate is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human CLEC4E Fc Chimera that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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