Recombinant Human CLEC9A Protein (Lys57-Val241), N-hFc-tagged

Product Description

Cat

IMP-10220

Official Symbol

CLEC9A

Product Overview

Recombinant human CLEC9a protein (Lys57-Val241) with a Human IgG1 (Pro100-Lys330) Fc tag at N-terninus was expressed in Mouse myeloma cell line.

Description

CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells.

Expression System

Mouse myeloma cell line

Species

Human

Tag

N-hFc

Predicted N Terminal

Met

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 47.8 kDa (monomer)
SDS-PAGE: 55-65 kDa, reducing conditions

Protein length

Lys57-Val241

Bio-activity

Measured by flow cytometry for its ability to bind a ligand expressed in FasL-treated mouse B cells. Sancho, D. et al. (2009) Nature 458:899. At 100 ng/mL, >50% of necrotic cells bind to Recombinant Human CLEC9a.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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