Recombinant Human CLEC9A Protein (Lys57-Val241), N-hFc-tagged

Product Description

Cat

IMP-10220

Official Symbol

CLEC9A

Product Overview

Recombinant human CLEC9a protein (Lys57-Val241) with a Human IgG1 (Pro100-Lys330) Fc tag at N-terninus was expressed in Mouse myeloma cell line.

Description

CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells.

Expression System

Mouse myeloma cell line

Species

Human

Tag

N-hFc

Predicted N Terminal

Met

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 47.8 kDa (monomer)
SDS-PAGE: 55-65 kDa, reducing conditions

Protein length

Lys57-Val241

Bio-activity

Measured by flow cytometry for its ability to bind a ligand expressed in FasL-treated mouse B cells. Sancho, D. et al. (2009) Nature 458:899. At 100 ng/mL, >50% of necrotic cells bind to Recombinant Human CLEC9a.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry