Recombinant Human CSF3R Protein, C-His-tagged

Product Description

Cat

IMP-4370

Official Symbol

CSF3R

Product Overview

Recombinant extracellular domain (Met 1-Pro 621) of human G-CSF receptor (NP_000751.1) precursor was fused with a polyhistidine tag at the C-terminus.

Description

Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.

Expression System

HEK293

Species

Human

Tag

C-His

Predicted N Terminal

Glu 25

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The mature recombinant human GCSFR consists of 608 amino acids and predicts a molecular mass of 68 kDa by SDS-PAGE under reducing conditions, the apparent molecular mass of rhGCSFR is approximately 92 kDa due to glycosylation.

Protein length

Met1-Pro621

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 85 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured by its ability to inhibit the GCSF-induced proliferation of NFS60 mouse myeloid cells.The ED50 for this ettect is typically 50-250 ng/mL in the presence of 0.125ng/mL of recombinant human GCSF.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry