Recombinant Human CSF3R Protein, No Tag
Product Description
Cat
IMP-4371
Official Symbol
CSF3R
Product Overview
The mature form of human G-CSF receptor (NP_000751.1) extracellular domain (Met 1-Pro 621) with five aminio acids at the C-terminus was expressed and purified.
Description
Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.
Expression System
HEK293
Species
Human
Tag
No Tag
Predicted N Terminal
Glu 25
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The mature recombinant human G-CSFR consists of 603 amino acids and predicts a molecular mass of 69.0 KDa. As a result of glycosylation, the apparent molecular mass of rhG-CSFR is approximately 94 KDa in SDS-PAGE under reducing conditions.
Protein length
Met1-Pro621
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 85 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-Cell based assay 1
Bioactivity-Cell based assay

Measured by its ability to inhibit GCSF-induced proliferation of NFS60 mouse myeloid cells. The ED50 for this effect is typically 2-8 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
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  • 1-631-559-9269
    1-516-512-3133
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