1 μg/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue Lane 1: non-reducing conditions Lane 2: reducing conditions
Serial dilutions of human CTGF, starting at 10 μg/mL, were added to HUVECs cultured without EGF. Cell proliferation was measured after 87 hours and the linear portion of the curve was us used to calculate the ED50.