Recombinant Human CTLA4 Protein (36-161aa), C-hIgG-His-Tagged

Product Description

Cat

IMP-9509

Official Symbol

CTLA4

Product Overview

Recombinant human CTLA4, fused to hIgG His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Description

CTLA-4, also known as cytotoxic T-lymphocyte protein 4, is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. The affinity of CTLA-4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

Expression System

Baculovirus

Species

Human

Tag

C-hIgG-His

Form

Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Molecular Mass

40.8 kDa (368 aa)

Protein length

36-161

AA Sequence

<ADL>KAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSD<LEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH>

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Applications

SDS-PAGE, Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5 mg/mL (determined by absorbance at 280nm)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Human CTLA-4 inhibits Human B7 1/CD80 induced IL-2 secretion of the Jurkat human acute T cell leukemia cells. The ED50 range ≤150 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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