Recombinant Human CTLA4 protein, C-Fc Tag

Product Description

Cat

IMP-254

Official Symbol

CTLA4

Product Overview

Recombinant Human CTLA4 protein(AA Ala 37-Ser 160)(Accession # NP_005205.2) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 39.9 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 90 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Ala 37-Ser 160

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>98% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity-ELISA 1

Immobilized Human B7-2, His Tag at 10 ug/mL (100 uL/well) can bind Human CTLA-4, Fc Tag with a linear range of 2-78 ng/mL.

Bioactivity-ELISA 2

Immobilized Human CTLA-4, Fc Tag at 10 ug/mL (100 uL/well) can bind Human B7-1, His Tag with a linear range of 0.156-1.25 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag: Biotinylated Human B7-1, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.8260 ug/mL.

Bioactivity-ELISA 4

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag: Biotinylated Human B7-2, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.1701 ug/mL.

Bioactivity-BLI 1

Loaded Human CTLA-4, Fc Tag on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 0.52 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human CTLA-4, Fc Tag on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 1.9 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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