Recombinant Human CTLA4 protein, C-Fc Tag
Product Description
Cat
IMP-254
Official Symbol
CTLA4
Product Overview
Recombinant Human CTLA4 protein(AA Ala 37-Ser 160)(Accession # NP_005205.2) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.
Expression System
HEK293
Species
Human
Tag
C-Fc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 39.9 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 90 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length
Ala 37-Ser 160
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>98% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Human CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human B7-2, His Tag at 10 ug/mL (100 uL/well) can bind Human CTLA-4, Fc Tag with a linear range of 2-78 ng/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Immobilized Human CTLA-4, Fc Tag at 10 ug/mL (100 uL/well) can bind Human B7-1, His Tag with a linear range of 0.156-1.25 ug/mL.

Bioactivity-ELISA 3
Bioactivity-ELISA

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag: Biotinylated Human B7-1, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.8260 ug/mL.

Bioactivity-ELISA 4
Bioactivity-ELISA

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag: Biotinylated Human B7-2, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.1701 ug/mL.

Bioactivity-BLI 1

Loaded Human CTLA-4, Fc Tag on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 0.52 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2
Bioactivity-BLI

Loaded Human CTLA-4, Fc Tag on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 1.9 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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