Recombinant Human CTLA4 protein, C-hFc Tag
Product Description
Cat
IMP-6010
Official Symbol
CTLA4
Product Overview
Recombinant Human CTLA4 protein(P16410)(37-162aa), fused with C-terminal hFc tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-hFc Tag
Form
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Molecular Mass
42.5 kDa
Protein length
37-162aa
AA Sequence
AMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSDF
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Purity
Greater than 91% as determined by SDS-PAGE.
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized CD152 at 2 μg/ml can bind Anti-CD152 rabbit monoclonal antibody, the EC50 of human CD152 protein is 27.14-34.82 ng/ml.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized CD152 at 2 ug/ml can bind Anti-CD152 rabbit monoclonal antibody, the EC50 of human CD152 protein is 27.14-34.82 ng/ml

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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