Recombinant Human CTLA4 protein, C-hFc Tag
Product Description
Cat
IMP-6010
Official Symbol
CTLA4
Product Overview
Recombinant Human CTLA4 protein(P16410)(37-162aa), fused with C-terminal hFc tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-hFc Tag
Form
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Molecular Mass
42.5 kDa
Protein length
37-162aa
AA Sequence
AMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSDF
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Purity
Greater than 91% as determined by SDS-PAGE.
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized CD152 at 2 μg/ml can bind Anti-CD152 rabbit monoclonal antibody, the EC50 of human CD152 protein is 27.14-34.82 ng/ml.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized CD152 at 2 ug/ml can bind Anti-CD152 rabbit monoclonal antibody, the EC50 of human CD152 protein is 27.14-34.82 ng/ml

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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