Recombinant Human CTLA4 protein, C-His-Avi Tag, Biotinylated

Product Description

Cat

IMP-634

Official Symbol

CTLA4

Product Overview

Biotinylated Recombinant Human CTLA4 protein(Accession # Q6GR94-1)(Ala 37-Phe 162), fused to C-His-Avi Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 17.2 kDa. As a result of glycosylation, the protein migrates as 22-30 kDa under reducing (R) condition, and 37-45 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Ala 37-Phe 162

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Human B7-1, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, His,Avitag with a linear range of 0.7-13 ng/mL.

Bioactivity-ELISA 2

Quantitative Analysis of CTLA-4 x OX40 Bispecific Antibody in Human Serum by Intact Assay. Immobilized Human OX40 Protein, His Tag (MALS verified) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 bispecific antibody in 50% Human serum and then add Biotinylated Human CTLA-4, His, Avitag at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL.

Bioactivity-SPR 1

Biotinylated Human CTLA-4, His,Avitag captured on Biotin CAP-Series S sensor Chip can bind Yervoy (Ipilimumab) with an affinity constant of 0.635 nM as determined in a SPR assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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