Recombinant Human CTLA4 protein, C-His-Avi Tag, Biotinylated

Product Description

Cat

IMP-634

Official Symbol

CTLA4

Product Overview

Biotinylated Recombinant Human CTLA4 protein(Accession # Q6GR94-1)(Ala 37-Phe 162), fused to C-His-Avi Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 17.2 kDa. As a result of glycosylation, the protein migrates as 22-30 kDa under reducing (R) condition, and 37-45 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Ala 37-Phe 162

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Human B7-1, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, His,Avitag with a linear range of 0.7-13 ng/mL.

Bioactivity-ELISA 2

Quantitative Analysis of CTLA-4 x OX40 Bispecific Antibody in Human Serum by Intact Assay. Immobilized Human OX40 Protein, His Tag (MALS verified) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 bispecific antibody in 50% Human serum and then add Biotinylated Human CTLA-4, His, Avitag at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL.

Bioactivity-SPR 1

Biotinylated Human CTLA-4, His,Avitag captured on Biotin CAP-Series S sensor Chip can bind Yervoy (Ipilimumab) with an affinity constant of 0.635 nM as determined in a SPR assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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