Recombinant Human CTLA4 protein, C-His Tag

Product Description

Cat

IMP-255

Official Symbol

CTLA4

Product Overview

Recombinant Human CTLA4 protein(AA Ala 37-Phe 162)(Accession # NP_005205.2) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.4 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.

Protein length

Ala 37-Phe 162

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CTLA-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human B7-1, Fc Tag (HPLC-verified) at 2 ug/mL (100 uL/well) can bind Human CTLA-4, His Tag with a linear range of 0.2-4 ng/mL.

Bioactivity-ELISA 2

Immobilized Human B7-2, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CTLA-4, His Tag with a linear range of 1-6.4 ng/mL.

Bioactivity-SPR 1

Yervoy (Ipilimumab, Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human CTLA-4, His Tag with an affinity constant of 25.7 nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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