Recombinant Human CTLA4 protein, C-mFc Tag

Product Description

Cat

IMP-253

Official Symbol

CTLA4

Product Overview

Recombinant Human CTLA4 protein(AA Ala 37-Phe 162)(Accession # NP_005205.2) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.6 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100 kDa under non-reducing (NR) condition.

Protein length

Ala 37-Phe 162

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

mmobilized Human B7-1, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.1-2 ng/mL.

Bioactivity-ELISA 2

Immobilized Human B7-2, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.5-7.8 ng/mL.

Bioactivity-ELISA 3

Immobilized Ipilimumab at 5 ug/mL (100 uL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.1-0.8 ng/mL.

Bioactivity-BLI 1

Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 0.52 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 1.6 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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