Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
mmobilized Human B7-1, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.1-2 ng/mL.
Immobilized Human B7-2, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.5-7.8 ng/mL.
Immobilized Ipilimumab at 5 ug/mL (100 uL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.1-0.8 ng/mL.
Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 0.52 uM as determined in BLI assay (ForteBio Octet Red96e).
Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 1.6 uM as determined in BLI assay (ForteBio Octet Red96e).