Recombinant Human CTLA4 protein, C-mFc Tag
Product Description
Cat
IMP-253
Official Symbol
CTLA4
Product Overview
Recombinant Human CTLA4 protein(AA Ala 37-Phe 162)(Accession # NP_005205.2) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.
Expression System
HEK293
Species
Human
Tag
C-mFc Tag
Form
Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.6 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100 kDa under non-reducing (NR) condition.
Protein length
Ala 37-Phe 162
Endotoxin
Less than 0.1 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

mmobilized Human B7-1, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.1-2 ng/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Immobilized Human B7-2, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.5-7.8 ng/mL.

Bioactivity-ELISA 3
Bioactivity-ELISA

Immobilized Ipilimumab at 5 ug/mL (100 uL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.1-0.8 ng/mL.

Bioactivity-BLI 1

Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 0.52 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2
Bioactivity-BLI

Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 1.6 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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