Recombinant Human CX3CL1 Protein (Gln25-Arg339, Ser199Asn), C-6×His-tagged
Product Description
Cat
IMP-10232
Official Symbol
CX3CL1
Product Overview
Recombinant human CX3CL1/Fractalkine protein (Gln25-Arg339, Ser199Asn) with 6×His tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
No results obtained: Gln25 predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 35 kDa
SDS-PAGE: 90 kDa, reducing conditions
Protein length
Gln25-Arg339, Ser199Asn
Bio-activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED50 for this effect is 20-100 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CX3CR1. The ED50 for this effect is 2.5-10 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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