Recombinant Human DKK3 Protein (Ala22-Ile350), C-10×His-tagged

Product Description

Cat

IMP-9649

Official Symbol

DKK3

Product Overview

Recombinant human Dkk-3 protein (Ala22-Ile350) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-10×His

Predicted N Terminal

Ala22

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 37.5 kDa
SDS-PAGE: 64-74 kDa, reducing conditions

Protein length

Ala22-Ile350

Bio-activity

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Hsieh, S.Y. et al. (2004) Oncogene 23:9183. The ED50 for this effect is 3-12 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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