Recombinant Human DLL4 Protein (Ser27-Pro524), C-10×His-tagged

Product Description

Cat

IMP-9689

Official Symbol

DLL4

Product Overview

Recombinant human DLL4 protein (Ser27-Pro524) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-10×His

Predicted N Terminal

Ser27

Form

Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and PEG3350 with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 55.6 kDa
SDS-PAGE: 60-70 kDa, reducing conditions

Protein length

Ser27-Pro524

Bio-activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 150-600 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in sterile PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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