Recombinant Human DLL4 Protein (Ser27-Pro524), C-10×His-tagged

Product Description

Cat

IMP-9689

Official Symbol

DLL4

Product Overview

Recombinant human DLL4 protein (Ser27-Pro524) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-10×His

Predicted N Terminal

Ser27

Form

Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and PEG3350 with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 55.6 kDa
SDS-PAGE: 60-70 kDa, reducing conditions

Protein length

Ser27-Pro524

Bio-activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 150-600 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in sterile PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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