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Measured by its binding ability in a functional ELISA. When Recombinant Human Notch-1 Fc Chimera is coated at 250 ng/mL (100 μL/wll), Biotinylated Recombinant Human DLL4 Fc Chimera Avi-tag protein binds with an ED50 of 0.012-0.12 μg/mL.