Recombinant Human DLL4 Protein (Ser27-Pro524), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10035

Official Symbol

DLL4

Product Overview

Biotinylated recombinant human DLL4 protein (Ser27-Pro524) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Ser27

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 83 kDa
SDS-PAGE: 94-112 kDa, under reducing conditions

Protein length

Ser27-Pro524

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Notch-1 Fc Chimera is coated at 250 ng/mL (100 μL/wll), Biotinylated Recombinant Human DLL4 Fc Chimera Avi-tag protein binds with an ED50 of 0.012-0.12 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry