Recombinant Human DLL4 Protein (Ser27-Pro524), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10035

Official Symbol

DLL4

Product Overview

Biotinylated recombinant human DLL4 protein (Ser27-Pro524) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Ser27

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 83 kDa
SDS-PAGE: 94-112 kDa, under reducing conditions

Protein length

Ser27-Pro524

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Notch-1 Fc Chimera is coated at 250 ng/mL (100 μL/wll), Biotinylated Recombinant Human DLL4 Fc Chimera Avi-tag protein binds with an ED50 of 0.012-0.12 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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