Recombinant Human DPP4 Protein (Asn29-Pro766), C-6×His-tagged
Product Description
Cat
IMP-9946
Official Symbol
DPP4
Product Overview
Recombinant human DPPIV/CD26 (High Purity Dimer) protein (Asn29-Pro766) with a C-terminal Asp-Ile and 6×His tag was expressed in Mouse myeloma cell line.
Description
The DPP4 gene encodes dipeptidyl peptidase 4, which is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic type II transmembrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Dipeptidyl peptidase 4 is highly involved in glucose and insulin metabolism, as well as in immune regulation. This protein was shown to be a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV), and protein modeling suggests that it may play a similar role with SARS-CoV-2, the virus responsible for COVID-19.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
Asn29
Form
Supplied as a 0.2 μm filtered solution in MES and NaCl.
Molecular Mass
Predicted Molecular Mass: 86 kDa
SDS-PAGE: 95-110 kDa, reducing condtions
Protein length
Asn29-Pro766
Bio-activity
Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/μg as measured under the described conditions.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Noncovalently-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Bioactivity-ELISA 1
Enzyme Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/μg as measured under the described conditions.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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