Recombinant Human DPP4 Protein (Asn29-Pro766), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10059
Official Symbol
DPP4
Product Overview
Biotinylated recombinant human DPPIV/CD26 protein (Asn29-Pro766) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
The DPP4 gene encodes dipeptidyl peptidase 4, which is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic type II transmembrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Dipeptidyl peptidase 4 is highly involved in glucose and insulin metabolism, as well as in immune regulation. This protein was shown to be a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV), and protein modeling suggests that it may play a similar role with SARS-CoV-2, the virus responsible for COVID-19.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Asn29
Form
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 114 kDa
SDS-PAGE: 114-134 kDa, under reducing conditions.
Protein length
Asn29-Pro766
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant MERS-CoV Spike RBD His-tag Protein is immobilized at 1.00 μg/mL, 100 μL/well, Biotinylated Recombinant Human DPPIV/CD26 Fc Chimera Avi-tag binds with an ED50 of 10.0-80.0 ng/mL. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >2700 pmol/min/μg, as measured under the described conditions.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant MERS-CoV Spike RBD His-tag Protein is immobilized at 1.00 μg/mL, 100 μL/well, Biotinylated Recombinant Human DPPIV/CD26 Fc Chimera Avi-tag binds with an ED50 of 10.0-80.0 ng/mL. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >2700 pmol/min/μg, as measured under the described conditions.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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