Recombinant Human DPP4 Protein (Asn29-Pro766), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10059
Official Symbol
DPP4
Product Overview
Biotinylated recombinant human DPPIV/CD26 protein (Asn29-Pro766) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
The DPP4 gene encodes dipeptidyl peptidase 4, which is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic type II transmembrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Dipeptidyl peptidase 4 is highly involved in glucose and insulin metabolism, as well as in immune regulation. This protein was shown to be a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV), and protein modeling suggests that it may play a similar role with SARS-CoV-2, the virus responsible for COVID-19.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Asn29
Form
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 114 kDa
SDS-PAGE: 114-134 kDa, under reducing conditions.
Protein length
Asn29-Pro766
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant MERS-CoV Spike RBD His-tag Protein is immobilized at 1.00 μg/mL, 100 μL/well, Biotinylated Recombinant Human DPPIV/CD26 Fc Chimera Avi-tag binds with an ED50 of 10.0-80.0 ng/mL. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >2700 pmol/min/μg, as measured under the described conditions.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant MERS-CoV Spike RBD His-tag Protein is immobilized at 1.00 μg/mL, 100 μL/well, Biotinylated Recombinant Human DPPIV/CD26 Fc Chimera Avi-tag binds with an ED50 of 10.0-80.0 ng/mL. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >2700 pmol/min/μg, as measured under the described conditions.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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