Recombinant Human EFNA4 Protein (Leu26-Gly171), C-Fc and 6×His-tagged, Biotinylated Conjugated

Product Description

Cat

IMP-10089

Official Symbol

EFNA4

Product Overview

Biotinylated recombinant human Ephrin-A4 protein (Leu26-Gly171) with a human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Description

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin that has been implicated in proliferation and metastasis of several types of cancers.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc and 6×His

Predicted N Terminal

Leu26

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 43.7 kDa (monomer)
SDS-PAGE: 50 kDa, reducing conditions

Protein length

Leu26-Gly171

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized rmEphA7/Fc Chimera at 2 μg/mL (100 μL/well) can bind biotinylated rhEphrin-A4/Fc Chimera with a linear range of 0.16-10 ng/mL. Optimal dilutions should be determined by each laboratory for each application.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute with sterile PBS at 100 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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