Recombinant Human EGFR Protein (25-645), His-tagged

Product Description

Cat

IMP-8097

Official Symbol

EGFR

Product Overview

Human EGFR (P00533-1, 25 a.a.-645 a.a.) partial recombinant protein with His tag was expressed in Sf9 insect cells.

Description

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Expression System

Insect cells

Species

Human

Tag

His

Form

Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 μg/mL.

Molecular Mass

About 80 kDa

Protein length

25-645

AA Sequence

LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPS

Endotoxin

< 0.2 EU/μg of protein (gel clotting method)

Purity

> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.

Applications

Functional Study; SDS-PAGE

Storage

Store at 4 centigrade for 1 week. For long term storage store at -20 to -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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